[Management of pre-analytical nonconformities at the Microbiology Laboratory of CHU Ibn Rochd-Casablanca [between 2016 and 2018]]

The pre-analytical phase is one of the main sources of non-compliance [NC]. The objective of this work is to evaluate the NCs of the pre-analytical phase at the Microbiology laboratory of Ibn Rochd-Casablanca University Hospital, and to propose preventive solutions. It's a retrospective descriptive study that involved 2028 cases of pre-analytic NC seized over a period of 24-month [August 2016 -July 2018]. This study was based on the exploitation of the computerized data base of our laboratory. From 56143 analysis requests processed during the 24 months, 2028 [3,6%] cases of pre-analytical NC were reported. The most pronounced dysfunctions were noted the pediatric services [15%]. The control of the pre-analytical phase is an important point to reduce sampling nonconformities. The collaboration between the various actors [prescribers, samplers and biologists], the establishment of a NC file, a sampling guide, continuous training and staff awareness are needed to improve the quality of this step

Epidemiological profile of invasive bacterial diseases in children in Casablanca, Morocco: antimicrobial susceptibilities and serotype distribution

The aim of this prospective study in Morocco was to investigate the causes of invasive bacterial diseases in children in order to inform antibiotic therapy and vaccine choices. Of 238 children aged

[Characteristics of Listeria monocytogenes isolates from Casablanca]

From January 1990 to December 1999 six isolates of Listeria monocytogenes were recovered in Casablanca from blood and / or CSF from 5 neonates and one three years old child. These isolates were studied by serotyping, phage typing and pulsed field gel electrophoresis. Resistance to 10 antibiotics was determined by E test method . All the isolates belong to serotype 1/2 b, except one serotype 4b. All the isolates were phage untypable. Four profiles were obtained by pulsed field gel electrophoresis . All the isolates were susceptible to penicillin G, ampicillin , gentamycin , rifampin and cotrimoxazole . One isolate [serotype 1/2b] was resistant to tetracycline [CMI > 256 mg/L] and one isolate [serotype 4b] was multiresistant [erythromycin MIC = 8 mcg/ml, chloramphenicol MIC > 256 mg/L and tetracycline MIC > 256 mg/L]. These results confirm the interest of DNA based techniques for typing sporadic isolates and stress the need for continuous surveillance of antibiotic resistance since multiresistance may occur

[E. Coil K1, neonatal infection]

Between January 1988 and December 1991. A study about 10 cases of E. Coli K1 neonates infection was taken. This study find high data of mortality and morbidity. We assure that rapid diagnosis is an important test